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KMID : 1094720190240040613
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Biotechnology and Bioprocess Engineering
2019 Volume.24 No. 4 p.613 ~ p.621
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Decreased Growth and Antibiotic Production in Streptomyces coelicolor A3(2) by Deletion of a Highly Conserved DeoR Family Regulator, SCO1463
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Jeon Jong-Min
Choi Tae-Rim
Lee Bo-Rahm
Seo Joo-Hyun
Song Hun-Suk
Jung Hye-Rim
Yang Soo-Yeon
Park Jun-Young
Kim Eun-Jung
Kim Byung-Gee
Yang Yung-Hun
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Abstract
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Streptomyces spp. have been isolated from different environmental niches and are known to exhibit diversity in secondary metabolism. They have complex regulation system to control secondary metabolism, however it is difficult to prioritize the importance of specific regulator among the thousands of global or cluster-situated genes that may regulate secondary metabolism in different Streptomyces strains. Here we suggest a simple homology-based selection method to find out important regulators by comparing seven Streptomyces strains finding highly homologous regulators in various Streptomyces strains and showed highly homologous 11 regulators containing four well known regulators such as BldM, IclR, WhiD and NdgR. Among various regulators, we showed a putative transcriptional regulator in Streptomyces coelicolor A3(2), SCO1463 playing a pivotal role in growth, antibiotic production (actinorhodin[ACT] and undecylprodigiosin [RED] production), and production/utilization of organic acids such as propionate and succinate by making comparisons between the deletion mutant and the wild type strain. Although high homology in various strains does not always mean the importance of a gene, we suggested a criterion on which regulator should be studied first and which would be more important among more than one thousand regulators.
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KEYWORD
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antibiotic production, DeoR family transcriptional regulator, Streptomyces coelicolor, organic acid uptake
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